Dissertation
Dissertation > Medicine, health > Internal Medicine > Endocrine diseases and metabolic diseases > Islet disease > Diabetes

Serum A-FABP Levels and Its Expression in Atherosclerotic Plaques of Diabetes Mellitus

Author SuiShaoHua
Tutor BaiJie
School Taishan Medical College
Course Internal Medicine
Keywords adipocyte fatty acid binding protein atherosclerosis diabetes
CLC R587.1
Type Master's thesis
Year 2011
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ObjectiveBy testing adipocyte fatty-acid binding protein in serum and atherosclerotic plaques of type 2 diabetes patients with/without atherosclerosis, to investigate the association of A- FABP with type 2 diabetes mellitus and atherosclerosis.MethodsExperiment I69 patients with type 2 diabetes mellitus(T2DM)were enrolled in the study . 37 male, 32 female , 31~77 age, disease duration 2 months~21years . According to atheroscleros- is, they were divided into diabetic non-atherosclerotic group(A group),which is 32 cases, 17 males and 15 females, mean age 52.0±9.6 years,mean disease duration 9.1±2.4 years and di- abetic atherosclerotic group(B group),which is 37 cases, 20 males and 17 females, mean a- ge 50.4±11.3 years, mean disease duration 10.2±2.1 years. diabetic atherosclerotic group an- d diabetic non-atherosclerotic group were matched in age and sex.at the same time, 43 patie- nts(C group) with atherosclerosis non-diabetes were enrolled in the study,22males and 21 f- emales, mean age 52.8±10.5 years,mean disease duration 9.4±3.1years. The control group is health check personnel(D group),31 cases, 16 males and 15 females, mean age 48.0±5.9 y- ears. Serum levels of A-FABP were measured by enzyme-linked immunosorbent assay.Experiment IIAtherosclerotic samples were obtained from 81 patients undergoing carotid endartere- ctomy (CEA) and amputation,among those samples,51 are from T2DM patients (E group), which patients are 28 males and 23females, mean age 57.2±12.2 years,mean disease dura- tion 16.9±4.6 years and 30 are from non-diabetic patients (F group), which patients are 16 males and 14 females, mean age 58.9±12.9 years,mean disease duration 18.1±5.7 years. The samples were distinguished into four pathological stages by two pathologists in a blinded m- ethod. Group E includes stageⅠ9 cases, stageⅡ10 cases, stageⅢ12 cases, stageⅣ20 cases, group F includes stageⅣ30 case . In addition,10 normal arteries as control group (G group) were obtained from patients undergoing amputation,which patients 5 males and 5 females, mean age 56.8±11.7 years,were not with diabetes, hypertension, coronary heart disease and cerebrovascular disease.Expression of A-FABP in atherosclerotic arteries were measured by two-step immunohistochemical method.400 high power and 5 fields were selectedrandom- ly,then the percentage of positive cells was calculated and named as A-FABP label index. All of the statistical data was processed with SPSS 13.0 software.ResultsExperiment IDiabetic have-atherosclerotic group, diabetic non-atherosclerotic group and atheroscl- erotic group compared with the control group, the serum levels of A-FABP were higher (P < 0.01). Diabetic have-atherosclerotic group were higher than diabetic non-atherosclerotic gr- oup and atherosclerotic group , atherosclerotic group compared with the diabetic group, the serum levels of A-FABP were higher (P < 0.01)Experiment IIIn the control arteries regularly arranged elastic fibers and SMC.In these atherosclerotic arteries,macrophage and SMC are seen accumulated largely under the endothelial.Immunohistochemical staining showed anti A-FABP is brown immune complex particles accumulated in the foam cell of normal arteries and atherosclerotic arteries. But it is striking differences between diabetic have-atherosclerotic group and control group. The number of control group is little,while diabetic have-atherosclerotic group is much(P <0.01).In different stages of diabetic have-atherosclerotic group the expression of A-FABP were significantly different (P<0.01). In the last stage of atheroselerosis,diabetic have-atherosclerotic group compared with the atherosclerotic group, expression of A-FABP in atherosclerotic lesions is no significant differences, not statistically differences. (P> 0.05)Conclusion1. Serum A-FABP are risk factors of diabetes.2. Serum A-FABP are risk factors of atherosclerosis.3.A-FABP take part in the development of diabetes and vascular complications .A-FABP levels in atherosclerotic lesions are associated with an serious plaque phenotype.4. There is not a necessary link between serum glucose levels and A-FABP levels in atherosclerotic lesions in the last stage.

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