Tupaia ( shrews ) cold-adapted and uncoupling protein 1
|School||Yunnan Normal University|
|Keywords||Tupaia ( ? ) Brown adipose tissue Uncoupling protein Cold acclimation Energy balance Thermogenic capacity Purification Enzyme Immunoassay|
This thesis is located in southern China, a typical tropical, subtropical small mammals, tree shrews (Tupaia belangeri), as the research object, from the individual level and at the molecular level of cold adaptation characteristics under low temperature stress research. Divided into four parts: 1. Cold acclimation in tree shrews energy metabolism; 2. Cold acclimation of tree shrews heat production capacities; 3. Solution the tree shrews brown adipose tissue uncoupling protein 1 purified; Rabbit anti-the tree shrews brown adipose tissue uncoupling protein-1 antiserum preparation and enzyme-linked immunoassay. The main findings and conclusions are as follows: 1. During cold acclimation in tree shrews weight significantly increased body temperature significantly reduced. The weight change tendency is first decreased and then increased. Tree shrews body temperature with cold acclimation time was a significant negative correlation. In the process of continuous cold stress, the animal body temperature after resistance to low temperature invalid eventually dropped that in the subtropical regions of the mammals have the ability to cold, but ultimately can not survive in low temperature environment for long periods. 2. Cold acclimation have a significant impact on the energy balance of tree shrews. After 28 days of cold acclimation, tree shrews food intake (dry weight) a significant increase in energy intake, digestible energy assimilation digestibility and assimilation rate, and body heat value significantly increase fecal excretion (dry The material weight) was significantly reduced, and the manure thermal value also significantly reduced. This series of physiological adaptation characteristics at low temperatures eventually associated with the ability to improve the cold-adapted, and increase the ability to survive in the environment of low temperature stress. This resistance to low temperature stress to spend large amounts of energy are closely related. 3. Cold acclimation in tree shrews heat production capacities significantly. Continuing cold acclimation process, the tree shrews RMR, NSTmax, CIRMR significantly increased. The high proportion of tree shrews RMR increase than the typical small mammals in northern, northern typical and NSTmax increasing Chuan than small mammals, CtoWKA4R ratio is decreasing. Show that the main memory in the south of the tropical and subtropical small mammals do not need * S ** to increase due to resistance to low temperature ribs. Forced by the additional consumption of energy, mainly by increased RMR thermogenic properties of cold-adapted and continuous cold stress environment by increased CllLMJI to improve its low temperature stress tolerance. 4. In Lin staple al (198) from the rodent purified the UCPI the method based on the successful from Tupaia Ju brown adipose tissue be isolated and purified the UCP successfully established so that the Tupaia the species separation and Purification UCPI the method. Of Tupaia Pina BAT UCPI molecular weight 33.142kDa, and composed of about 306 amino acid residues, and other mammals UCPI on the structure and function may be very similar. At the same time, measuring the impact of cold acclimation ** PI content of brown adipose tissue. The cold acclimation 28 days of UCP 'content, the content of mitochondrial protein, and brown adipose tissue weight increased significantly. That cold ribs, forcing the process Tupaia thermogenic capacity and energy metabolism is closely related to the increase uncoupling protein content changes on a molecular level. 5. Successfully prepared rabbit anti the Tupaia 嗣 brown adipose tissue UCP antiserum, to prepare rabbit anti-UCP antiserum in Tupaia threshold of the species. Successfully established enzyme-linked rabbits infected analysis, and the use of this method to detect the presence of the UCP in brown adipose tissue, but not in the liver and muscle; liver and muscle may to exist UCPZ and UCP3 and UCPZ the content ratio of the high UCP3 content. The results showed that the UCP is mainly distributed in the brown adipose tissue, this result and Cannon, et al. The same conclusion. These results suggest that, under the influence of a sustained cold stress, the typical tropical, subtropical small mammals its cold adapt to the heat production characteristics in order to gain weight, lower body temperature, increased energy storage, to reduce energy discharged adaptation model to enhance the ability to resist low temperature stress. Meanwhile, in the level of biological macromolecules take the corresponding mechanisms to carry out the effective energy distribution and the energy balance. Tupaia brown adipose tissue distribution UCP, liver and muscle may the distribution UCPZ and UCP3.