Dissertation
Dissertation > Medicine, health > Oncology > Gastrointestinal Cancer > Gastric neoplasms

A Comparative Study of the Gastric Fluid Proteomes from Gastric Cancer Patients and Normal People

Author FuBingJie
Tutor HuangShiAng
School Huazhong University of Science and Technology
Course Immunology
Keywords Gastric cancer Gastric juice Proteomics Biomarker
CLC R735.2
Type Master's thesis
Year 2009
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Gastric cancer is the most common malignant tumor of the Asia-Pacific region , and is the third most important cause of death in cancer . Gastric cancer patients with early subclinical , and current detection methods (endoscopy , biopsy ) sensitivity is not high enough , to make gastric cancer has not been effective early diagnosis . Gastric cancer biomarker looking for specific ideas to solve this problem , but has yet to find a sensitive and specific biomarkers . The gastric juice has a strong organ specificity therefrom proteome may find clinical application of early diagnosis of gastric cancer markers can be successfully realized . Objective : In this study, the gastric juice physico-chemical properties ( initial acidity , color ) may be caused by gastric juice proteome gel electrophoresis with style , and this impact and gastric cancer is a preliminary study for the further use of mass spectrometry (MS ) , liquid chromatography (LC) mining gastric juice gastric cancer protein markers in the foundation . Method: 1. Collect process samples , preliminary statistical analysis of the sample information ; 2 . Preliminary analysis of the potential link between the the gastric juices initial acidity and gastric by polyacrylamide gel electrophoresis (PAGE) experiments , as well as its possible influencing factors . Results and discussion : the distribution of the sex and age of the gastric cancer samples collected in the experiment in line with the reported data ; 2 . Initial pH value of gastric juice may be related to the anatomical location of gastric cancer ; 3 . Gastric juice samples gel electrophoresis styled by the influence of the initial pH value : the initial pH value of 1 to 2 , by the strip less , more complete separation , and the initial pH value ≥ 6 by strip number is more dense , and some in the former the uniform density appears strip not evident in the latter , or the concentration of large differences . The possible causes of pepsin in alkaline conditions to generate and active suppression , low-abundance protein concentration is not degradation , and gastric cancer or precancerous lesions of the gene expression changes , lead to further change in the protein composition of gastric juice .

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