The Relation between Expression of IL-8、 ICAM-1 in Tissue Surrounding the Hematoma and Brain Edema in Experimental Intracerebral Hemorrhage and the Intervention Role of Injection of Ginkgo Biloba Leave Extract
|School||Ningxia Medical University|
|Keywords||Cerebral hemorrhage Ginkgo biloba extract injection Rats Interleukin-8 Intercellular adhesion molecule-1|
Objective To observe the rats after intracerebral hemorrhage the hematoma surrounding tissue IL-8, ICAM-1 expression and its relationship with cerebral edema explore the extract of Ginkgo biloba the injection (Ginaton) the protection of the inflammatory response in rat brain hemorrhage role. The caudate nucleus injection of heparinized collagenase copy cerebral hemorrhage animal model, and give Ginaton intervention. 160 SD rats were randomly divided into five groups: sham operation group, model group and Ginna tall, three lower dose groups Each group was divided into 6 h, 24 h, 3 d, 7 d4 point in time, each time point eight rats. Signs of neurological deficit score Bederson score, with the determination of the water content of the brain tissue wet / dry weight method using HE staining under an optical microscope to observe the pathological changes of the brain tissue around the hematoma, immunohistochemical methods intracerebral hemorrhage surrounding tissue in IL-8, ICAM-1 expression. Results Ginaton neurological score intracerebral hemorrhage in rats: cerebral hemorrhage and neurological score in rats increased significantly, appear obvious neurological deficit. 24 h after cerebral hemorrhage, the most obvious (P lt; 0.01), a the Ginna tall, middle dose group can reduce the rat nerve function score (P lt; 0.05 or P lt; 0.01). The Jenner multiple low-dose group also to some extent reduce the neurological score after cerebral hemorrhage, but at various time points with the model group showed no significant difference (P gt; 0.05); ginaton on intracerebral hemorrhage brain edema: the rat brain 24 h after hemorrhage, brain water content began to increase peaked (P lt; 0.01), 3 d to 7 d less back to normal. Ginna tall, brain water content of the medium dose group compared with the model group decreased, the difference was significant (P lt; 0.05) Ginaton low dose group in brain water content is reduced to a certain extent, but at each time point. compared with the model group, there was no significant difference; Ginaton pathological morphology around the hematoma after intracerebral hemorrhage in rats: intracerebral hemorrhage in rats perihematoma significant inflammatory response, manifested as bleeding after 6 h shows a small amount of a single scattered inflammatory cell infiltration, infiltration of inflammatory cells 24 h mainly neutrophils significantly increased 72 h visible around the hematoma and hematoma glial cells, vascular proliferation, 7 d hematoma was significantly reduced, glial cells and vascular marked hyperplasia. Jenner edema of how high-dose group than in the low-dose group edema was mild malacia of the brain tissue was significantly reduced, and a large number of capillary proliferation; Ginaton perihematoma lL-8 content intracerebral hemorrhage in rats: sham operation around the needle tract tissue of rats can be seen a small amount of IL-8 immunoreactive cells, microvascular endothelial cells; model group 6 h after hematoma surrounding tissue with a small amount of IL-8-positive cells, mainly expressed in vascular endothelial cells, glial cells , neurons, 24 h, reached a peak (P lt; 0.01), 3-d when the number of positive cells decreased 7 d still a small number of positive cells. Ginaton dose group rats perihematoma IL-8 immune response compared with the model group over the same period the number of positive cells reduced significantly the dose the greater the effect of the more obvious; Ginaton cerebral hemorrhage rat ICAM-1 perihematoma the impact of content: sham group needle tract surrounding tissue can be seen a small amount of ICAM-1 positive staining of blood vessels, and no expression of neurons. Model group each phase point of the the hematoma surrounding tissue have different levels of ICAM-1 positive blood vessels and nerve cells, with the sham group than the existence of a significant difference (P lt; 0.01). 6 h after cerebral hemorrhage surrounding tissue expression to 3 d reached a peak, and continue until 7 d still expressed. Each dose group at each time point perihematoma Ginaton ICAM-1 content were lower (P lt; 0.05 or P lt; 0.01), among which the least Jenner how much dose group; brain tissue ICAM-1, IL-8 content changes and brain water content relative relationship: changes in the level of IL-8 and ICAM-1 levels and brain water content trends. Conclusion cerebral hemorrhage, hematoma surrounding tissue ICAM-1, IL-8-mediated inflammatory cell infiltration, expression of inflammatory cytokines and brain edema and cerebral hemorrhage on time. Prompted both may be relevant to the inflammatory response after cerebral hemorrhage; changes in IL-8 levels of ICAM-1 levels and brain water content of the same trend, indicating a cerebral edema development may be relevant; Ginaton can improve mouse neurological score, reduce cerebral edema, inhibition of inflammatory cell infiltration, inhibition of cerebral hemorrhage, IL-8, and ICAM-1 expression, blocked the inflammatory response, the protective effect of intracerebral hemorrhage, and its protective effect a dose-dependent manner.