Establish a New Model of Abdominal Aortic Aneurysms and to Study the Relationship of NOS with the Formation of AAA
|Keywords||abdominal aortic aneurysm nitricoxide synthase experimental model rabbit|
Objective: Abdominal aortic aneurysm (abdominal aortic aneurysm, AAA) is the most common lesions of the aortic expansion. The incidence of the disease has increased year by year. And the specific mechanism for its formation is not clear. Now it is considered that the formation of abdominal aortic aneurysm is the anatomical, genetic, environmental, biochemical and hemodynamic changes in a variety of factors, such as results. With in-depth study, the role of the nitric oxide synthase in aortic aneurysm arouse researchers more and more attention. Through the establishment of this experimental animal model of abdominal aortic aneurysm, a new method,make a direct result of rabbit abdominal aorta , and detect the nitric oxide N synthase (i-NOS) activity on artery wall in normal and aneurysmal tissue ,to explore the changes of the inducible nitric oxide synthase (iNOS) expression and its relationship to abdominal aneurysm formation.Methods: During the operation of the experiment group,the vascular prosthesis of (stuff) PTFE was implanted to the rabbit abdominal aorta to form an aneurysm, while sham operation was done in the control group. The tissue of abdominal aortic aneurysm was harvested in1d, 7d,14d, and 28d after operation, respectively. The tissue bomogenate concentration of iNOS in the abdominal aortic aneurysm were detected by enzyme linked immunosorbent assay (ELISA).Results: In experimental group, mean concentration of the tissue bomogenate concentration of iNOS in the abdominal aortic aneurysm in 1-day after operation、7-day after operation、14-day after operation、28-day after operation is 22.129±2.518 u/ml、27.337±5.321 u/ml、36.047±4.584 u/ml、44.756±1.799 u/ml,respectively,In control group,that is 12.499±1.807 u/ml.The concentration of iNOS in experimental group is significantly higher than that of control group,(each P<0.01). The difference during the experimental group all have statistical significance,(each P<0.05); In experimental group the diameter of the abdominal aortic aneurysm is significantly higher than that of control group,( P<0.01). The difference during the experimental group all have statistical significance,( P<0.05); However, in normal group the diamete between eath group isn’t statistically significant (P> 0.05). There is positive correlation between the concentration of iNOS and the diameter of the abdominal aortic aneurysm in experimental group. (r=0.896 ,p<0.01).Conclusion: 1.)The concentration of iNOS in organization of each experimental group were significantly higher; The tumor diameter of the experimental group is significantly higher than that in the control group.2.) The concentration of iNOS in the tissue of experimental group rabbit abdominal aortic aneurysm is positively correlated with the tumor diameter.3.) The comparison of iNOS in the tumor tissue of each experimental group is significantly different, and the concentration of iNOS increased ,with the increase of abdominal aortic aneurysm’existence in time.4.) The iNOS may play an important role in the formation and progression of the abdominal aortic aneurysm.