STUDIES ON THE SYSTEM OF MATURE EMBRYO CULTURE IN VITRO OF RICE TRANSGENE
|Course||Crop Genetics and Breeding|
|Keywords||transgene rice restorter of three lines mature embryo culture callus induction green plantlet regeneration culture ability culture system|
Some principal factors affecting the efficiency on mature embryo culture of rice were studied systematically on restorter of rice three-lines in this paper. The results indicated that:1. The effects on mature embryo sterilization were quite different with different sterilant combinations. 70% CH3CH2OH 3 min + 10% NaCIO 10 min + 0.1% HgCl2 15 min had optimal effect on mature embryo sterilization.2. Basic media were selected in mature embryo culture. NM medium was adaptable better to callus induction. Full quality of basic media were more suitable to callus subculture than half quality of that. But MS basic medium was advantageous to plant regeneration of mature embryo.3. Study showed that proper concentration of Amino Acid in callus induction medium could increase the rate of callus induction and further improve the ability of mature embryo culture. Different Amino Acid categories and concentrations in callus induction medium showed higher frequency in mature embryo culture than single Amino Acid category and concentration.4. Increasing the Vitamin concentration in appropriate range could improve the frequency of callus induction and green plantlet differentiation. Twice Vitamin concentration in callus induction medium showed the best effect in the test.5. The new type of hormone-Thidiazuron (TDZ) could suppress the callus induction but promote plant regeneration in mature embryo culture.6. 2, 4-D was a necessary factor in callus induction. 2.0mg/L 2,4-D in callus induction medium was optimal. 2,4-D combined with KT/BA/NAA in induction media could expressthe better efficiency on mature embryo culture.7. The effects of callus induction, subculture and differentiation were quite complex according to different hormone categories, concentrations and their combinations.8. There were different effects with different genotype varieties and different inoculation seasons on mature embryo culture.9. 100-120mg/L Vitamin C (Vit C) was added to subculture medium could decrease the callus browning.10. The rate of green plantlet differentiation was to the maximum after callus induction for 17 days, subculture for 10-15 days.The rate was decreased with the prolongation time for the subculture and the subculture times increase.11. Both callus induction media and callus subculture media had obvious residual action on effecting the differentiation of green plantlets. Using the induction media, subculture media and differentiation media of mature embryo callus reasonablly could improve the culture ability effectively.12. There were different requirements with different hormone categories, concentrations and combinations in the test on root induction and sound seeding of embryo plant.