Studies on the Characters of Digestive Enzymes in Scophthalmus Maximus
|School||Ocean University of China|
|Keywords||Turbot Digestive enzymes Protease Amylase Lipase|
In this paper, the digestive enzymes of the progress of the domestic and foreign fish, turbot (Scophalmus maximus) digestive enzymes from the perspective of Biochemistry nature. Of Turbot under ① farming water temperature of digestive enzymes distribution and various digestive enzymes optimum temperature optimum pH of. ② main chemical reagents turbot digestive activity. ③ the use of the nondenaturing electrophoresis separation of turbot digestive enzymes. ④ different growth stages of turbot digestive enzymes change. The results are as follows: 1, the turbot digestive system is divided into 5 parts of the stomach, pyloric caeca, foregut, midgut and hindgut. Various parts of the protease activity in the optimum pH, temperature, followed by 40 ° C, 2.0; 40 ° C, 8.0; 60 ° C, 8.0; 40 ° C, 8.5; 60 ° C, the rearing water temperature of 4.0 ° C 18 ° C, in a suitable pH range, protease activity from high to low for pyloric caeca> stomach> foregut, the midgut hindgut. The amylase activity of the various parts of the optimum pH, temperature, followed by 40 ° C, 6.5; 40 ° C, 8.0; 40 ° C, 7.5; 50 ° C, 7.0; 40 ° C, 8.0.18 ° C rearing water temperature, in a suitable pH range amylase activity from high to low pyloric blind sac> foregut> intestine> intestine> stomach. Lipase activity of the various parts of the optimum pH, temperature, followed by 40 ° C, 7.5; 40 ° C, 6.5; 40 ° C, 7.0; 40 ° C, 7.5; 40 ° C, the rearing water temperature of 7.5 ° C 18 ° C, in a suitable pH range lipase activity from high to low for foregut midgut pyloric caeca> intestine> stomach. 2, enzymatic analysis method research EDTA, NaCl 11 kinds of metal ions (Cu 2 sup> Mg 2 sup> Fe 3 sup>, Hg 2 sup>, Zn 2 sup>, Ca 2 sup>, Pb 2 sup>, Cd 2 sup>, Ag sup> and Mn 2 sup>) of turbot pepsin, foregut protease, pyloric caeca amylase and foregut fat enzyme. EDTA concentration is set to 0.2 × 10 -3 sup> mol / L, 0.5 × 10 -3 sup> mol / L, 1 × 10 -3 sup> mol / L, 2 × 10 -3 sup> mol / L concentration, NaCl concentration is set to 0.05 mol / L, 0.1 mol / L, 0.2 mol / L, 0.5mol/L4 a concentration of 11 kinds of metal ion concentrations were set to 5 × 10 -3 sup> mol / L concentration. The experiments show that EDTA, NaCl, no significant effect of protease, Ca 2 sup> Mg 2 sup>, the Fe 3 sup> activated protease, Cu < sup> 2 sup>, Hg 2 sup>, Ag sup> have different degrees of inhibition of protease; Ca 2 sup>, Mn 2 sup> activation of amylase the EDTA, NaCl, Cu , 2 sup> Zn 2 sup> of Cd 2 sup> the Pb 2 sup>, Hg 2 sup>, Mg 2 sup> amylase inhibition; of NaCl, K sup> Ca 2 sup > activation of lipase Cu 2 sup> Mg 2 sup> the Fe 3 sup>, Hg 2 sup> Zn < sup> 2 sup>, Pb 2 sup>, Cd 2 sup>, Ag sup> of Mn 2 sup> lipase inhibition. 3, turbot age for the study of material, non-denaturing electrophoresis turbot digestive enzymes preliminary separation. Using gelatin in situ protease digestion, detected six enzyme with pepsin under acidic conditions, which have a strong belt, two strong band, three weak bands: pyloric caeca protease detected four enzymes with which two strong bands, a strong band, a weak band; the foregut protease detected three enzyme bands, of which there are two strong bands, a weak band, and the three enzymes with pyloric caeca protease The first three enzymes with the same molecular weight level, but the activity of lower; detect midgut protease enzyme with two weak; hindgut detected a weak enzyme with protease and midgut protease with on the level of the same molecular weight. Amylase, amylase, gastric amylase l in strong enzyme bands detected; The pyloric caeca amylase detected four enzymes with, three strong bands, a weak band: the foregut amylase detected two enzymes with a strong band, l weak with; midgut, hindgut amylase each detected a weak band: and stomach, midgut, hindgut amylase with pyloric caeca foregut the second strip in the same molecular weight level. Isoenzyme law lipase lipase, gastric lipase enzyme bands detected four, a strong band, a strong band and two faint bands; the pyloric caeca lipase detect gastric lipase enzyme band; foregut lipase detected five enzymes with two strong bands, a strong band and two weak bands; detect midgut lipase enzyme with 5 l strong with two strong band , two weak band. The hindgut lipase detection with intestinal lipase enzyme with just each one with active smaller; digestive system of the various parts of the lipase with the same molecular weight level. 4, Turbot Na digestion enzyme type and vitality size has changed with the growth and development of the fish. The turbot drunk intestinal proteinase activity change: In the protease activity of the five growth stages upward trend from maximum age varies, but the growth rate of the growth phase; protease activity increased 30-day to 90-day-old The fastest, at 90 days to 180 days of age, the growth rate is slightly faster, tl-day-old to slower growth in an age, an age until age rarely, an age of fish with age fish protease activity was essentially flat. Fresh turbot gastrointestinal amylase activity change: amylase activity was increased and then decreased trend in five growth stages, activity reached the maximum of 180 days; fastest the proteinase activity growth rate of 30-day to 90-day-old, 90 days of age to 180 days of age, the rate of growth slows down, the enzyme activity began to decline in the 180 days of age to age, an age until age continue to decline. The turbot Na gastrointestinal lipase activity change: lipase activity was gradually rising trend in five growth stages, from the age maximum.