A Primary Study on in Vitro Selection of Hydroxyproline-resistant Mutants of Eggplant
|School||Huazhong Agricultural University|
|Keywords||Eggplant Cold Tolerance Somaclonal variation Hydroxyproline Mutant|
Eggplant (Solanum melongena L.) is a nutrient-rich, unique flavor and strange medicinal value of vegetables, and therefore popular. In recent years, with the expansion of eggplant consumption increasing and eggplant protected cultivation area, the needs of special varieties of eggplant protectorate increasingly strong, the protected cultivation varieties resistant to cold, resistant to low light characteristics was not very prominent. Thus breed resistant to cold, low-light protection of special varieties of resistance is very necessary. Due to the continuous promotion of hybrid varieties, resulting in the loss of many local varieties, which gradually narrows the genetic background of the eggplant. Eggplant cultivars not yet looking to the Cold Tolerance of planting materials, it is difficult to breed cold-resistant varieties through conventional methods. Means of somatic cell fusion, transgenic technology has bred some of the cold-resistant varieties, use of clonal variation has also been successful in some crops. Variability in plant tissue culture regenerated plants often very extensive, and the variation of the frequency is very high. It can happen at the chromosomal level, and can also occur at the molecular level. People somaclonal variation combined with pressure screening has fostered many disease-resistant, resistance inverse varieties. In vitro culture techniques in conjunction with the laboratory conditions, the study by establishing a set of eggplant, then the in vitro selection screen to the cold-resistant callus and regenerated plants. The main results are as follows: 1. Add eggplant hypocotyls and cotyledons of NAA and BA growth regulator on MS medium induced callus, but a significant variety of callus induction rate differences between the genotypes. Different concentration combinations of BA and NAA of eggplant callus induction has an important impact, 2.0mg / L BA 0.2mg / L NAA induced callus more closely, pale yellow to pale green, is conducive to the differentiation. 2. Different genotypes of eggplant callus differentiation of adventitious buds, but the differentiation rate is not the same. Despite the different genotypes of eggplant callus out are not the same the best adventitious buds hormone combination, 2.0mg / L to 4.0 mg / L of BA on callus differentiated adventitious buds to play an important role. 10mg / L AgNO 3 to promote adventitious buds differentiation also plays a supporting role. 3. Adventitious buds or without addition of 0.2mg / L NAA 1/2MS medium can grow roots, 2 to 3 weeks after carefully transplanted to pots survived cultured under 28 ℃ and high humidity conditions . 4. Callus after subculture adapter adding different concentrations of hydroxyproline (L-Hyp) subculture medium. With the increase of the concentration of L-Hyp, the survival rate of callus is gradually decreased. The survival rate of only 4.3% when the concentration of 8 mmol / L: the callus not survive when further improve. Therefore, 8 mmol / L L-Hyp for screening pressure, is expected to screening of the mutant anti-L-Hyp. 5. With 8 mmol / L L-Hyp repeated screening, resistant callus and regenerate plants. Physiological and biochemical characteristics of cold tolerance, resistant callus proline content and soluble sugar content was significantly higher than that of the control, MDA content and conductance percentage was significantly lower than that of the control, and thus better cold tolerance . Proline content of the regenerated plants was significantly higher than the control, conductance percentage was significantly lower than that of the control, showing greater resistance to cold.