Experimental study of gastric the Ning side of SGC-7901 human gastric cancer cell lines

Objective: To explore traditional Chinese medicine gastric cancer rather the mechanism of action of the human gastric cancer cell line SGC-7901, to provide a reliable basis for the traditional Chinese medicine clinical application of treatment of gastric cancer. METHODS: SGC-7901 human gastric cancer cell lines in vitro, plus serum of gastric Ning. HE staining and light microscopic enzyme cytochemical staining, electron microscope technology to observe the morphological changes of gastric Ning serum of gastric cancer cell line SGC-7901 and activity changes. Results: (1) enzyme cytochemistry the drug serum after SGC-7901 human gastric cancer cell plasma membrane flag enzyme magnesium - adenosine triphosphatase (the Mg ^{2 -ATPase), the flag of the endoplasmic reticulum enzyme glucose-6 - phosphatase (G-6-PASE) reaction particles are smaller, significantly reduced the number to reduce the density, and some negative. The enzyme activity decreased significantly. Chinese medicine group and control group significant differences (P <0.05). (2) electron microscope medicine group SGC-7901 cell ultrastructure changes microvilli and protrusions significantly reduced, the pseudopodia disappear, mitochondrial vacuolation. Nuclear chromatin condensation, apoptotic bodies. (3) the role of drug serum for 24 hours SGC-7901 human gastric cancer cells significantly inhibited the inhibition rate was 47.78%: for 48h inhibition is more obvious inhibition rate was 61.88%, the inhibitory effect was time-dependent. Is not the same phase serum on proliferation of SGC-7901 human gastric cancer cells, to the best 3D-2t-2h group of drugs serum inhibition, inhibition rate of 66.41%. Conclusion: 1, Chinese gastric cancer rather reduce the human gastric cancer cell line SGC-7901 magnesium plasma membrane marker enzyme - adenosine triphosphatase (the Mg 2 -ATPase), the endoplasmic reticulum marker enzyme glucose-6 - phosphatase ( G-6-Pase) activity. 2, Chinese medicine gastric Ning inhibit SGC-7901 human gastric cancer cell proliferation and induce apoptosis in cancer cells. 3, 3d-2t-2h drug serum, as the study of traditional Chinese medicine rather inhibition of gastric cancer SGC-7901 human gastric cancer cell proliferation more reliable method of serum pharmacology. 4, Chinese medicine rather reduce gastric cancer SGC-7901 cells invasion and metastasis. Preface the anti-cancer effects of Chinese medicine has been the compelling subject of the medical profession. More than in the past from the perspective of elimination of tumor to find treatment options. In recent years, the anti-cancer treatment in China attaches importance to study traditional Chinese medicine, Western medicine in the treatment of cancer their own characteristics, play traditional Chinese medicine, director of cancer efficacy has been significantly improved. The same time, related to traditional Chinese medicine and cancer research further depth. Gastric ranked second in the incidence of cancer around the world, gastric cancer can occur at multiple ages, the age of onset of gastric cancer in China between the ages of 40-50 is the most common, accounting for about 2/3 of the total number of cases. Gastric cancer is a traditional Chinese medicine, nausea, belch choking, epigastric pain, accumulation of disease areas. The incidence of gastric cancer is related to many factors, many studies have shown that, in addition to the changes in cell cycle regulation, apoptosis regulation disorders also lead to tumor cell hyperplasia of the important reasons. In recent years, a large number of experimental studies have found that gastric cancer cells can be cleared by artificially triggering apoptosis. Traditional Chinese medicine-induced apoptosis provides a new approach for the treatment of gastric cancer in the future. Gastric cancer rather than a single traditional Chinese medicine are more likely factors on apoptosis interference and influence. Help to clarify the TCM syndrome differentiation and the overall concept of Chinese medicine theory, cells}