Studies on the Active Constituents of Asparagus Cochinchinensis
|School||Second Military Medical University|
|Keywords||Aspartame Furostanol saponins Antifungal activity The anti-tumor activity Cardiovascular and cerebrovascular activity Antifibrotic activity|
Tuber aspartame (Asparagus cochinchinensis) Liliaceae (Liliaceae) plant aspartic (Asparagus cochinchinensis (Lour.) Merr.), A traditional Chinese medicine. Widely distributed in the country, is mainly produced in Guizhou, Guangxi, Yunnan, Gansu, Anhui, Henan, Hunan, Hubei, Sichuan, Jiangxi and other places are also produced. Aspartame is only contained in the \Its sweet, bitter, cold,, normalized lung and kidney by. For the 2000 edition of \Dryness of dry cough is mainly used in the treatment, Dayton sputum sticky throat, thirst, intestinal dry constipation embolism. Looking for anti-oxidation, anti-aging active ingredients from natural products has become a hot topic of current drug research. Pharmacological studies have shown that aspartame the chloroform extract, alcohol extract and water extract has antioxidant effects of different levels of galactose induced aging mice is not clear, but the material basis of the antioxidant effect of aspartame the chemical composition studied inadequate, only reported that some amino acids, monosaccharides, oligosaccharides, polysaccharides, and a few saponins compounds, therefore, we think, both from the point of view of basic research or applied research, there is a need to take advantage of modern separation chemical aspartame and identification techniques to systematically study its activity screening, in order to find the active ingredient or lead compounds for the aspartame research and development to provide the scientific basis. The ethanol extract production from Hubei Huangshi aspartame isolated 30 compounds were elucidated by spectroscopic methods (IR, UV, MS, 1H-NMR, 13C-NMR and 2D-NMR) and chemical methods (hydrolysis, sugar nitrile acetate-GC), to determine the structure of which thirteen compounds were, respectively: AC1: β-sitosterol alcohol in AC2: β-daucosterol AC3: a positive - thirty-two carbonated AC4: palmitic acid AC5: 9 - heptadecene AC6: sarsasapogenin AC7: diosgenin AC8: Smilax sapogenin-3-O-[α-L-rhamnose pyranose-(1-4)]-β-D - Grape pyranoside AC9: (25S)-26-O-beta-D-grape pyranose yl-5β-the furostanol -20 (22) - ene-3β, 26 - diol-3-O-α- L-rhamnopyranosyl-(1-4)]-β-D-glucopyranoside AC10: (25R)-26-O-of β-the D-grape pyranose group - furostanol -5,20 - diene-3β, 26 - diol LT; WP = 6 GT;-3-O-[alpha-L-rhamnose pyranose group (1-2)] - [(β-D-grape pyranose - (1-4))-α-L-rhamnose pyran glycosyl - (1-4) - β-D-glucopyranoside Model AC11: (25S)-26-O-of β-the D-grape pyran the glycosyl-5β-Furostanol -20 (22) - ene-3β, 15,26 - triol-3-O-alpha-L-rhamnose pyranose group (1-4)]-β-D-grape pyranoside The AC12: (25R)-26-O-beta-D-grape pyranose yl-5β-the furostanol -20 (22) - ene-3β, 26 - diol-3-O-[β-D the - grape pyranosyl - (1-2)]-β-D-glucopyranoside. AC13: (25R)-26-O-of β-the D-grape pyranose group - furostanol -5,20 - bis ene-3β, 26 - diol-3-O-alpha-L-rhamnose pyranosyl-(1-2)] - [α-L-rhamnose pyranose group - (1-4)] - β-D-glucopyranoside compound, AC9 and AC10, Model AC11 The AC12 is a new compound, and the remaining compounds are the first time from the plant except AC1, AC13 share. Identification of some small amount of structure of the compound is still in progress. Pharmacological experiments show that AC8 with antifungal activity and anti-tumor activity, AC8 Candida albicans MIC value 8μg/ml.; Tetrazolium salt (Methyl-Thiazol-Tetrozolium, MTT) reduction AC8 in HL-60 human leukemia cell line has a certain activity, inhibition rates were 48 hours: 100% (10-5mol / l), 41.9% (10-6mol / l); through sulfonyl Rhodamine B (sulforhodamine B, SRB) protein staining AC8 breast cancer cell line MDA-MB-468 has a certain activity, 72 hours inhibition rates were: 99.4% (10-4mol / l), 99.3% (10-5 mol / l); aspartame total saponins improve anesthesia the activity of the the canine brain blood flow in the cerebral vascular resistance and reduce the heart rate of anesthetized dogs, AC9 improve cerebral blood flow in anesthetized dogs and reduce the activity of the cerebral vascular resistance is also very significant, in this study we have applied for a patent. Total saponins another aspartame has a significant inhibitory effect on hepatic stellate cells HSC-T6 and proliferation of NIH-3T3 fibroblasts, the inhibition rate of HSC-T6 were: 13.42% (13μg/ml), 15.49% (104μg / ml); inhibition rate of NIH-3T3 were: 22.06% (26μg/ml), 37.47% (104μg/ml). The activity of the compounds of the remaining monomer being studied. By orthogonal experiments of this study on aspartame Extraction Process optimization, to determine the best extraction process: aspartame coarse to eight times the amount of 75% ethanol extraction, vacuum recovery to no alcohol taste, diluted with water macroporous resin column chromatography, 30% ethanol, collected 80% ethanol elution part of total saponins vacuum recovery of ethanol. The study also established HPLC-ELSD method of total saponins active ingredient AC9 content determination, quality control based on for aspartame herbs and preparations.