Dissertation > Medicine, health > Chinese Medicine > Of Pharmacy > Pharmacology > Chinese medicine Experimental Pharmacology

The Protein Expression of S180 Mice P53 Caused by Oleanolic Acid the Effective Ingredient of Ligustrum Iucidum and the Induction of TNF-α

Author GaoMing
Tutor WuBoYan
School Heilongjiang University of Traditional Chinese Medicine
Course Chinese and Western medicine combined with the basis
Keywords Ligustrum Oleanolic acid Antitumor P53 Tumor Necrosis Factor
CLC R285.5
Type Master's thesis
Year 2010
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Objective: To explore the active ingredients of Chinese medicine Ligustrum at the molecular level oleanolic acid anti-tumor effect of S180 tumor-bearing mice mechanism . Method : Copy S180 mice model , inhibitory rate was calculated and the life span ; using protein electrophoresis technique to observe tumor protein and serum protein bands change ; immunohistochemical method to observe the expression of P53 protein and tumor necrosis factor . . Results: oleanolic acid inhibitory effect , inhibition rate was 48.59% , the life extension rate of 44.62% , and no significant side effects . Oleanolic acid S 180 tumor-bearing mouse tumor protein and serum protein expression of certain bands . Oleanolic acid can down-regulate the expression of mutant p53 protein in tumor cells , cells of tumor necrosis factor- induced effects , compared with model group , the difference was statistically significant ( P lt; 0.01 ) . Conclusion : Ligustrum oleanolic acid , the active ingredient can effectively inhibit tumor growth and prolong the survival time of tumor-bearing mice . Serum protein and tumor tissue of tumor-bearing mice protein expression of certain bands . Oleanolic acid can affect the expression of the P53 protein , tumor cells and tumor necrosis factor . Experiments prove that the the Ligustrum lucidum active ingredients of oleanolic acid has good in vivo anti- tumor effect .

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