Dissertation
Dissertation > Medicine, health > Chinese Medicine > Of Pharmacy > Pharmacology > Chinese medicine Experimental Pharmacology

The Ameliorative Effect and Mechanism of Procyanidins Extracted from the Lotus Seedpod on Age-associated Memory Impairment in Rats

Author RongShuang
Tutor LiuLieGang
School Huazhong University of Science and Technology
Course Nutrition and Food Hygiene
Keywords Lotus seedpod proanthocyanidins Scopolamine Mice Spatial learning and memory ability Morris water maze Jumping AChE LSPC Learning and Memory Morris water maze Oxidant antioxidant Cholinergic system
CLC R285.5
Type Master's thesis
Year 2009
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Part I: scopolamine made memory impairment model mice test part (lotus seedpod proanthocyanidins on scopolamine-induced memory impairment in role) Objective: To observe the lotus seedpod proanthocyanidins on scopolamine-induced learning and memory impairment. Methods: 50 male Kunming mice were randomly divided into five groups, each group of 10 normal control group, model group, lotus seedpod proanthocyanidins low dose group (L-LSPC, 50 mg / kg), the lotus seedpod proanthocyanidins dose Group (M-LSPC, 100 mg / kg), lotus seedpod proanthocyanidins high dose group (H-LSPC, 150 mg / kg). Three dose groups were given a dose lotus seedpod proanthocyanidins intragastrically for 30 days, during which the normal group and model group was given the same amount of saline. Before each test was given 10 min scopolamine intraperitoneal injection (1 mg / kg, ip) induced memory impairment in mice models, behavioral experiments (Morris water maze' dissertation">Morris water maze, jumping method) for indicators observed lotus seedpod proanthocyanidins barriers to learning and memory in mice role. Acetyl cholinesterase AChE activity observed changes in the mice in each group cholinergic system and through the brain tissue was detected. Results: with respect to the model control group mice, the lotus original anthocyanins can significantly improve the scopolamine induced memory obstacle mice in the Morris water maze performance, significantly shortening its incubation period, and reduce their swimming distance; jumping experiments can extend the platform to stay period, and a significant reduction in the number of errors. And lotus seedpod proanthocyanidins can inhibit scopolamine-induced elevated AChE activity. Conclusion: lotus seedpod proanthocyanidins obvious improvement effect of scopolamine-induced learning and memory impairment. One of the mechanisms may be based on its impact on the cholinergic system. The second part of the test portion of the aged rats (lotus seedpod proanthocyanidins memory impairment disorders affect learning in old rats and its possible mechanisms) Section I: lotus seedpod proanthocyanidins elderly memory loss disorder rats purpose of learning and memory in: by Morris water maze test, observation lotus seedpod proanthocyanidins rat spatial learning and memory ability in elderly learning and memory disorders. : Morris water maze in aged rats filter, 4-month-old young rats latency, selected from 200 18-month-old elderly SD (Sprague-Dawley) female rats learning and memory impairment in rats and senile memory in normal rats. Senile memory impairment rats were randomly divided into three groups: namely senile memory disorder group (AI), the lotus seedpod procyanidins low dose group (L-LSPC, 50 mg / kg) and high dose group (H-LSPC, 100 mg / kg); 4-month-old SD rats normal rat and senile memory at the same time as the young control group (young) and old age memory is normal control group (AU). L-LSPC and H-LSPC groups were given a dose lotus seedpod proanthocyanidins intragastrically seven weeks, meantime elderly normal group and youth group was given the same amount of saline. Impact observed by Morris water maze of proanthocyanidins for elderly patients with memory disorders rat spatial learning and memory ability. Results: Compared with the AI ??rats, L-LSPC and H-LSPC were able to significantly improve spatial learning and memory abilities senile memory impairment in rats, the performance of the L-LSPC and H-LSPC rats in the Morris water maze latency was significantly shortened significantly reduced swimming distance; H-LSPC group two latency and swimming distance indicators with no significant difference in the youth group and the AU group. Conclusion: lotus seedpod proanthocyanidins on the memories of old rats diminished barriers to improvement. Section II lotus proanthocyanidins for elderly patients with memory loss obstacles oxidation antioxidant levels in rat brain tissue Objective: To observe the lotus seedpod proanthocyanidins senile memory disorders oxidation antioxidant levels in the rat hippocampus and cerebellum. : Detect the rat hippocampus and cerebellum, total antioxidant capacity (total antioxidant capacity, TAO-C), malondialdehyde (malondialdehyde, MDA), the level of the antioxidant glutathione (glutathione, GSH), anti- oxidase mitochondrial superoxide dismutase (superoxide dismutase, SOD), catalase (catalase, CAT), glutathione peroxidase (glutathione peroxidase, GPx) and other activity, and protein carbonyl content, to observation lotus seedpod proanthocyanidins senile memory disorders improved oxidation antioxidant levels in the brain tissue of rats. Results: Compared with young rats, the degree of oxidative damage in the brain tissue of aged rats AU and AI groups significantly increased. Was no significant difference between the AU group and the AI ??group. Compared with the AU and AI groups of aged rats, L-LSPC and H-LSPC were able to significantly reduce the degree of oxidative damage in rat brain senile memory disorders. Showed that L-LSPC and H-LSPC group rat brain tissue MDA levels decreased TAO-C levels increased. And H-LSPC group change is more significant, dose-dependent relationship. 3 compared with aged rats AU and AI groups, L-LSPC and H-LSPC able to significantly improve the AI ??rat brain tissue levels of antioxidants and antioxidant enzyme activity. And H-LSPC group change is more significant, dose-dependent relationship. Conclusion: lotus seedpod proanthocyanidins may be to reduce the level of oxidative stress within brain tissue damage and improve the level of antioxidants and antioxidant enzymes play its role in the protection of the aged rat brain tissue. The third quarter lotus seedpod proanthocyanidins senile memory loss obstacles cholinergic system in the brain tissue of rats Objective: To observe the lotus seedpod proanthocyanidins for elderly patients with memory disorders cholinergic system in the rat hippocampus and cerebellum. Methods: detect the rat hippocampus and cerebellum acetylcholine ACh content, acetylcholinesterase AChE and acetyl choline the enzyme ChAT activity, to observe lotus seedpod proanthocyanidins obstacles cholinergic system for elderly patients with memory loss. Results: 1 AU group and youth group AI rats ACh in the hippocampus and cerebellum was significantly decreased compared to L-LSPC and H-LSPC can significantly improve the the older memory impairment in the rat brain ACh content, no significant difference in the AU group and youth group, and H-LSPC group change is more significant, dose-dependent relationship. 2 ChAT activity was no significant difference between the groups. Compared with the youth group, AI rats hippocampal and cerebellar tissue AChE activity was significantly reduced, L-LSPC and H-LSPC can significantly improve the activity of AChE in senile memory impairment in rat brain tissue, and H-LSPC group The change is more significant, dose-dependent relationship. Conclusion: LSPC by reversing the reduction of AChE activity, improve the level of ACh improve elderly and memory impairment, cholinergic system in the brain tissue of rats, thus improving learning and memory and cognitive ability.

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