Study on the Methods of Quality Assessment and Pharmacokinetics of Changbitong Granule
|School||Shenyang Pharmaceutical University|
|Keywords||The smooth nose through particles Quality Control Methods Cinnamic acid Pharmacokinetics|
Smooth nose through particle (Ning Jin granules) received are set out in the traditional Chinese medicine ministerial standard 17 by twig, Angelica, Nepeta, wind, skullcap, white peony root, mint and licorice and so on Bawei herbs, has to reconcile business health solutions Table of scattered wind effect, mainly used for air-dry cold cold, Camp David is not and evil wind, sweat, headache, sneezing, nasal congestion and allergic rhinitis: urticaria and other allergic disorders see the syndrome. The smooth nose through particles of the research object, on the basis of the original standard to improve the smooth particles nasal quality control methods, and the index components cinnamic acid pharmacokinetic studies in rats. Using thin-layer chromatography (TLC), and to identify the prescription windproof, angelica root, skullcap, Nepeta and licorice flavors herbs. High performance liquid chromatography (HPLC) method, cinnamic acid, baicalin, baicalein cohosh prime glycosides ,5-O-A 基维斯阿米 glycosides, paeoniflorin and glycyrrhizic acid content determination method, The linear range: 0.82 ~ 8.2μg · mL -1 sup> (r = 0.999 9), 31.0 ~ 310.0μg · mL -1 sup> (r = 0.999 9), 3.2 ~ 32.0μg · mL -1 sup> (r = 0.999 7), 2.4 ~ 24.0μg · mL -1 sup> (r = 0.999 9), 2.4 ~ 24.0μg · mI -1 sup> (r = 0.999 9), 10.8 ~ 108.0μg · mL -1 sup> (r = 0.999 8) and 7.0 to 56.0μg · mLq (r = 0.999 7) ; recoveries were: 101.1% (RSD = 2.2%), 100.5% RSD: 2.0%), 101.4% (RSD: 1.9%), 100.1% (RSD = 1.5%), 100.7% (RSD = 1.9%), 100.4% (RSD = 2.0%) and 99.4% (RSD = 1.9%). The results showed that the concentration and peak area of ??each component in the above range linear relationship well established method is simple, reproducible, and provides a basis for the evaluation of the quality of the smooth nose particles. Rats were given the the smooth nose through particles, plasma cinnamic acid content determination method. Chromatography for the conditions a Diamonsil TM sup> C 18 column (200 mm x 4.6 mm, 51 μm) column with methanol - acetonitrile -0.5% acetic acid (39:20:60, v / v / v) as the mobile phase flow rate of 1.0 mL · min -1 sup>, detection wavelength 280nm, column temperature of 35 ° C. The cinnamic acid linear range was 0.06 ~ 6.0μg · mL -1 sup> (r = 0.998 8), extraction recoveries were 84.9%, 82.1% and 87.0%, intraday and interday precision (RSD) are less than 5.8%. The method is specific, reproducible, easy to operate. The analysis method, cinnamic acid pharmacokinetic study in rats. Single dose in rats after oral administration of smooth nose through particles, the main pharmacokinetic parameters were: 0.87 ± 0.16 μg · mL -1 sup> the C max , T , max 0.167 ± 0.053h, t 1/2 8.62 ± 2.00h, Ke 0.085 ± 0.022 h -1 sup>, AUC 0-t 3.68 ± 1.51μg · h · mL -1 sup>, AUC 0 - ∞ 4.51 ± 1.54μg · h · mL -1 sup>.