Dissertation
Dissertation > Medicine, health > Chinese Medicine > Of Pharmacy > Pharmacology > Chinese medicine Experimental Pharmacology

The Effects and Mechanisms of Benefiting-bone Granule Against Osteoporosis

Author ZhangYing
Tutor XuHuiQin
School Nanjing University of Traditional Chinese Medicine
Course Pharmacology
Keywords Yigu particles Analgesic Anti-inflammatory Mice Mesenteric microcirculation Ovariectomized Bone density Bone metabolism Serum pharmacology Proliferation of osteoblasts
CLC R285.5
Type Master's thesis
Year 2009
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Chapter analgesic, anti-inflammatory effects.: The observed Yigu particles analgesic and anti-inflammatory effects. Methods: analgesic experimental mice were randomly divided into five groups. (1) blank control group: distilled water; (2) Yigu particle low-dose group: 8g raw drug / kg; (3) Yigu particles in dose group: 16g raw drug / kg; (4) Beneficial bone particles high dose group: 32g crude drug / kg; (5) bone Gusongbao group: 2g/kg. Each group were gavage administration dose volume 0.2ml/10g. The analgesic effect of the hot plate test and acetic acid writhing the observation Yigu particles. The anti-inflammatory effects in three ways - mouse peritoneal capillary permeability induced by intraperitoneal injection of acetic acid induced mouse ear edema, croton oil mixed proinflammatory agents, plastic ring granulation rats test swollen impact. The first two anti-inflammatory the experiment and analgesic experiments like grouping administration, the the third experimental rats were randomly divided into five groups. (1) blank control group: distilled water; (2) Yigu particle low-dose group: 4g raw drug / kg; (3) Yigu particles in dose group: 8g raw drug / kg; (4) Beneficial bone particles high dose group: 16g crude drug / kg; (5) bone loose the Po group: 1g/kg. 10 days after modeling ig volume of administration to once a day. At the same time, once a day for consecutive 14 days. Results: Yigu particles each dose group at all time points after administration pain threshold were higher than the blank control group, especially in the hour after administration of analgesic effect is most obvious. The beneficial bone particles in the high-dose group significantly reduced acetic acid induced writhing number of times, compared with the blank control group, a significant difference (P <0.05, 0.01). The particles of Yigu suppression induced by acetic acid in mouse peritoneal capillary permeability increased (P <0.05, 0.01). Beneficial bone particles each dose group could reduce the mouse ear swelling, compared with the control group, a significant difference (P <0.05, 0.01). The Yigu particles the dose group granuloma NW, compared with the control group, there were significant differences (P <0.05, 0.01). Conclusion: Yigu particles have some analgesic and anti-inflammatory effects. The the second chapter Yigu particles mouse mesenteric microcirculation Objective: To observe of particles Yigu mouse mesenteric microcirculation. METHODS: Kunming mice were randomly divided into five groups. (1) blank control group: distilled water; (2) Gusongbao group (GSB): 2g/kg; (3) Yigu particles low-dose group (BBG-L): the 8g Pharmacognostic / kg; (4) Yigu particles middle dose group (BBG-M): 16g crude drug / kg; (5) Yigu particles high dose group (BBG-H): 32g crude drug / kg. Each group were orally administered volume 12ml/kg, administered for three consecutive days. Fasting can not help but water for 12 hours before the experiment, after intragastric administration of sodium pentobarbital 40mg/kg, intraperitoneal injection of anesthesia, the back-bit fixed right lower abdomen shearing cut about 3cm incision, pulled out the period of bowel loops, flat Shop at plexiglass perfusion Koike, pins fixed 30 ° C slow perfusion magnifying glass 20 times magnification observation microvascular fixed field of view, as a cold light source with a high-pressure mercury lamp. Separation of the femoral vein supply medicinal, intravenous 10% the polymer dextran 6ml/kg, 10 minutes and 30 minutes respectively to observe and count recorded microcirculation indicators. The number of crossover points observed capillaries around the boundary of the fixed area within a fixed area, blood flow velocity, capillary blood flow state. Results: beneficial bone particles three dose groups capillary network crossover points are increased; blood speed faster, compared with the blank control group, there were significant differences (P <0.05); decline in bloodstream status integral. Conclusion: Yigu particles promote the the open capillaries role, can significantly increase the capillary blood flow velocity and improve the state of capillary blood flow. Chapter III benefits bone particles experimental rat model of osteoporosis Objective: To observe the particles on experimental osteoporosis Yigu. Methods: Female 24 weeks of age, 90 SD rats were randomly divided into 6 groups. Blank control rats for processing, the remaining rats were anesthetized by intraperitoneal injection dose of 3% solution of sodium pentobarbital 1ml/kg by dorsal, complete removal of both ovaries, hemostatic suture. Blank control group, only the removal of a small amount of fat tissue. Postoperative intramuscular penicillin saline 20,000 u / kg, 3 days. Feeding a week later, will ovariectomized rats were randomly divided into five groups, with the blank control group, for a total of six groups: (1) blank control group: given the same amount of distilled water; (2) model group: given the same amount of distilled water; ( 3) Beneficial bone particles low-dose group (BBG-L): give beneficial bone particles 4g raw drug / kg; (4) Yigu particles in dose group (BBG-M): give Yigu particles 8g raw drug / kg; (5) the beneficial bone particles high dose group (BBG-H): give Yigu particles 16g crude drug / kg; (6) positive control group (CEE3): nylestriol tablet dissolved in distilled water, ig 1mg/kg. In addition to the positive control group orally once a week, and the other groups were daily gavage once (1ml/100g). Femoral bone mineral density (BMD) was measured in the three months after gavage the of blood biochemical parameters and urine biochemical indicators, calcitonin (CT), osteocalcin (BGP) and estradiol (E 2 ) content, and scanning electron microscopy of rat femoral metaphyseal cancellous bone. Results: the femur model group was significantly lower than that of the control group (P <0.01). Low Yigu particles, BMD in the high-dose group compared with the model group increased, the which Yigu particles, the high-dose group with a significant difference (P <0.05, 0.01). Model group, serum calcium, phosphorus content was significantly lower than the control group (P <0.05), alkaline phosphatase, hydroxyproline content was significantly higher than the control group (P <0.01). The beneficial low bone particles, the calcium in the blood with the model group, the high-dose group compared to the increase, alkaline phosphatase compared with model group decreased, which in the high dose group with a significant difference (P <0.05, 0.01). The the beneficial low bone particles in serum phosphorus content of the high-dose group compared with the model group increased hydroxyproline content compared with the model group decreased, including high-dose group with a significant difference (P <0.05). Model group, urinary calcium and urinary phosphorus content was significantly higher than the control group (P <0.05). Low Yigu particles, in the high-dose group, urinary calcium and the urine phosphorus content compared with the model group decreased, including high-dose group with a significant difference (P <0.05, 0.01). The model group calcitonin (CT) and estradiol (E 2 ) was significantly lower than the control group (P <0.01). Beneficial low bone particles increased, the content of the high-dose group compared with the model group, the content of calcitonin (CT) in the high-dose group and model group with a significant difference (P <0.05, 0.01), estradiol (E 2 ) content is low, medium and high dose group and the model group, a significant difference (P <0.01). The model group osteocalcin (BGP) content with blank control group showed no differences. Low Yigu particles in the high dose group osteocalcin (BGP) content compared with the model group also was no significant difference. The model group bone trabeculae sparse thinner diameter than open marrow cavity. And Yigu particles, improved the high-dose group. Conclusion: Yigu particles can increase bone density in ovariectomized rats, improve the micro-structure of cancellous bone. Low Yigu particles in the high dose group of alkaline phosphatase and osteocalcin content lower than the model group, can reduce estrogen after the removal of the ovaries due to a sharp decline in the high rate of bone turnover, and effectively delay the incidence of osteoporosis. Particles on osteoblast Chapter Yigu purpose: use of serum pharmacology method to observe the beneficial bone particles containing serum in vitro proliferation of neonatal rat calvarial osteoblasts (OB) and osteoblasts secrete IL 6 and TNF-α. Methods: Female 12-month-old 80 SD rats were randomly divided into the saline group Yigu particles low dose (the 8g Pharmacognostic / kg), medium dose (16g crude drug / kg) and high dose (64g crude drug / kg) group, gavage once daily for 12 consecutive days, respectively, after administration 1h, 1.5h, 2h blood collection, separation of the serum, with serum containing of different concentrations Yigu particles culture neonatal SD rat skull OB MTT assay of cell proliferation . 30 SD rats were randomly divided into three groups (control group, the beneficial bone particles group and Neil estradiol group) (n = 10), the beneficial bone particles (BBG) group 64g crude drug / kg ig (CEE3), of nilestriol group 1mg/kg (nylestriol tablet has dissolved in distilled water) gavage blank group was given the same volume of saline. Gavage once a day for 12 consecutive days before the last administration, fasting 12 h, free access to water and 1.5 h after the last dose of ether anesthesia, orbital blood 3000r/min centrifugal 10 min, and the supernatant. Vitro isolated, cultured osteoblasts, using enzyme-linked immunosorbent assay, to detect osteoblast IL-6 and TNF-α levels. Results: after the last administration, the absorbance (A) value of 1.5 h of blood collection group was significantly higher than the other groups time (P <0.05), different doses of various concentrations of beneficial bone particles containing serum on OB proliferation in vitro were significantly role in promoting, wherein the dilution ratio of the high dose group, the best 20% of the serum concentration. Conclusion: Yigu particles containing serum has a significant role in promoting the OB proliferation; beneficial bone particles for OB Efficacy rat serum containing preparation conditions equivalent dose eight times for 12 consecutive days orally, last administration After 1.5 h of blood collection, serum added concentration of 20% is appropriate. The the Beneficial bone particles group and Nylestriol group, IL-6 and TNF-α is lower than the control group, the difference was statistically significant (P <0.01).

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