VDAC-1, a-enolase, SOD2, GSTP2 in TCM FD model Significance
|School||Huazhong University of Science and Technology|
|Course||Biochemistry and Molecular Biology|
|Keywords||Functional dyspepsia (FD) Quantitative PCR Acrid Bitter medicine WKN VDAC-1 α-enolase SOD2 GSTP2|
Functional dyspepsia (FD) , also known as non- ulcer dyspepsia is a common clinical disease , but also a set of causes not yet fully clear clinical syndrome, including persistent or recurrent upper abdominal discomfort, fullness, early satiety , chest pain and discomfort , nausea , vomiting, or other upper abdominal symptoms , lasting at least three months or more , but no evidence of local or systemic organic disease clinical syndrome . Chinese medicine will be divided into the following three functional dyspepsia card types: ① weak stomach type ② ③ incoordination between liver and spleen type . FD pathogenesis is not fully understood , the pathogenesis of FD currently considered the following factors , which may be the result of their combined effects , as gastrointestinal motility dysfunction, visceral hypersensitivity , excessive gastric acid secretion , Helicobacter pylori infection, psychosocial and fat diet. Because the etiology and mechanism of the complexity and ambiguity of FD treatment to bring some degree of difficulty. Acrid Bitter medicine and its representatives WKN treatment of functional dyspepsia with good clinical results, but also in clinical practice has been widely used . In our previous study, disharmony of liver and type FD rats had drawn its normal group, model group, treatment group, total protein, by comparing the proteomic techniques to identify the expression of multiple proteins in the FD large rat normal group, model group, treatment group differences . These differentially expressed protein spots identified by mass spectrometry as VDAC-1, GSTP-2, α-enolase, SOD2 and so on. This experiment will establish three kinds of FD syndrome animal model , traditional Chinese medicine after the intervention , extracting the normal group , model group and treatment group rat antral tissue total protein and total RNA, using western blotting techniques and fluorescent quantitation PCR technology on VDAC-1, α-enolase, SOD2, GSTP2 these four targets were studied.