Change of Endogenous Hydrogen Sulfide/Cystathionine γ-Lyase System in Acute Lung Injury Induced by Acute Pancreatitis in Rats
|Keywords||acute pancreatitis associated lung injury (APALI) cystathionine-γ-lyase (CSE) hydrogen sulfide TNF-α|
Objective By observing the change of cystathionine-γ-lyase (CSE) in the level of mRNA in lung and content of H2S in blood in rats with acute pancreatitis associated lung injury, combined with the change of TNF-αin the level of mRNA and pathological changes in lung tissue to explore the relationship between hydrogen sulfide / cystathionine -γ- lyase system and acute pancreatitis associated lung injury.Methods A totle of 54 SD rats were radomly divided into three groups. (1) AP group was induced by injection of sodium deoxycholate into the common bile duct. (2) Drug intervention group was made by intraperitoneal injection of PAG (PAG, propargylglycine, CSE inhibitor) on the basis of the AP group. (3) Control group was made by sham operation. At 6, 12 and 24 hours after operation, each lung specimen was given pathological examination by HE and fluorescence quantitative PCR detection of expression of CSE and TNF-αin the level mRNA. Blood specimen was analyzed by deproteinization for the content of H2S.Result (1) Control group showed normal lung tissue. In AP group, at 12h the gross specimen showed brown areas of atelectasis, and small quantity of pleural effusion. By light microscope, quantity of infiltration of inflammatory cells and exudates were visible in pulmonary interstitial, alveolar and bronchial. In drug intervention group, exudates and bleeding in alveolar and bronchial decreased. (2) In AP group, at 6h the expression of CSE mRNA reached peak and was higher than in other two groups(P<0.05). At 3h and 6h the relative expression level of CSE gene expression was higher than in control groups(P<0.05), and at 3h ,6h and 12h the relative expression level of TNF-αgene expression was higher than in control groups(P<0.05). In drug intervention group, at 3h and 6h the relative expression level of CSE gene expression was lower than in AP groups(P<0.05), and at 6h the relative expression level of TNF-αgene expression was lower than in AP groups(P<0.05). (3) In the AP group, at 3h, 6h and 12h the serum concentration of hydrogen sulfide was higher than in the control group(P<0.05). In drug intervention group, at 6h and 12h serum concentrations of hydrogen sulfide was lower than in AP group.Conclusion (1) In the early stage of AP, hydrogen sulfide / cystathionine -γ- lyase system participates in the pathological process of APALI and increases it. (2) Intraperitoneal injection of PAG can inhibit the sulfide / cystathionine -γ- lyase system in lung and improve pathology score of lung tissue. (3) The sulfide / cystathionine -γ- lyase system increasing APALI may be in connection with TNF-α.