RNA silencing hyaluronidase HYAL1 gene on human breast cancer cells malignant potential the impact of
|School||Chongqing Medical University|
|Course||Department of General Surgery|
|Keywords||Angiogenesis Invasive potential Cell Cycle Human breast cancer cells Small interfering RNA RNA interference Hyaluronidase gene Reverse transcription polymerase chain reaction Gene expression levels|
Objective: To investigate SiRNA-HYAL1 hyaluronidase human breast cancer cells HYAL1 gene expression . Methods: RT-PCR. Results : SiRNA-HYAL1 act on different levels of expression of hyaluronidase human breast cancer cell line MDA-MB-231, MDA-MB-453S, ZR-75-30 and ZR-75 significantly inhibited after hyaluronidase HYAL1 mRNA expression levels (P lt; 0.05). Conclusion : SiRNA-HYAL1 effect on human breast cancer cells can specifically inhibit the expression of an mRNA HYAL1 .