Chemical Constituents and Antioxidant of Pongamia Pinnata
|School||Guangxi Medical University|
|Keywords||Pongamia Chemical composition Flavonoids Flow injection chemiluminescence Antioxidant|
Pongamia pinnata Pongamia Pinnata (L.) Merr. ( Synonym of Pongamia glabra) is a the Mangrove plant legume Pongamia genus is in one of the five kinds of semi- mangrove plants in Guangxi has traditional medicinal value of plants . Foreign scholars to the compounds isolated from Pongamia main flavonoids, flavanone , chalcone and triterpene domestic Pongamia few reports , especially the leaves have not been reported . Starting from the raw materials easy to obtain and the plant protection point of view , we have chosen Pongamia pinnata leaves the study of the chemical composition and biological activity , through research , we hope to find out in China and abroad Pongamia leaf chemical composition and biological the activity of the similarities and differences , and to provide a theoretical basis for the rational development and utilization of Pongamia medicinal value . Pongamia leaf MEALS 75% ethanol extraction, and then after the petroleum ether, chloroform , ethyl acetate and n- butanol and the like polar solvent extraction , divided into four different polar fractions . Then by silica gel column chromatography , polyamide column chromatography, macroporous adsorption resin and dextran gel (Sephadex LH-20) , a variety of chromatographic separation means consists of 10 compounds were based on chemical and spectral the method determines a 9 wherein the structure is: n - dodecanol , dandelion terpene alcohols , Pongamia prime , stigmasterol , pongaglabrone pongachromene Pongamia flavin , Vitex prime , n- dodecanoic acid . Which n - dodecanol , dandelion terpene alcohol , vitexin being an thirty-two and carbonic acid isolated from this plant for the first time , in another compound structure is identified . Antioxidant Activity of Pongamia not yet reported flow injection chemiluminescence method , we made ??a study of the antioxidant activity of Pongamia pinnata leaves and stems , experimental results show that : Pongamia pinnata leaves and stems have antioxidant The activity leaves strong anti- oxidation activity in the stems, leaves and stems lipophilic parts of antioxidant activity is stronger than that of a water - soluble parts . The middle of the lipophilic parts of the chloroform extract (# 3 ) and leaves the ethyl acetate extracts ( # 4 ) the strongest antioxidant activity , their IC 50 are below the vitamin C , having the strongest anti oxidation. The experimental results suggest the Pongamia folk for the treatment of rheumatism , rheumatism pharmacological mechanism may be related to its antioxidant effects .