Research on the Endophytes in Ginkgo Biloba L. and Magnolia Officinalis Rehd. et Wils
|School||Chengdu University of Traditional Chinese Medicine|
|Keywords||Ginkgo Magnolia Endophytic fungi Ginkgolide Bacteriostatic Mannitol|
Endophytic fungi life refers to a period of time in its life cycle within the plant tissue, plant tissue did not cause obvious disease symptoms fungi. Recent studies indicate that, endophytic fungi can produce the same or similar with host metabolites. This feature makes the endophytic fungi from new resources to find novel structure activity loyal and the important compounds. Thus, the topic chosen Ginkgo Ginkgo biloba L. and Magnolia Magnolia officinalis Rehd et Wils as the representative of the material to carry out the microbiology of the two plants endophytic fungi; ginkgo lactone activity strains screening studies; Magnolia antibacterial activity screening studies of the strains; the Magnolia active strains substance basic research work. Ginkgo root and stem of four different sources, a total of 178 endophytic fungi isolated identified by morphological observation and 1 class 4 orders, 6 families, 28 genera. Dominant populations in the the purpose classification level on Cong Terrier spore head (Moniliales) dominant populations; Fusarium (Fusarium) on the classification level of the genus Aspergillus (Aspergillus), Cladosporium (Cladosporium). It was found that the distribution of the number and types of endophytic fungi of Ginkgo different parts are quite different, from different sources ginkgo similar situation. Reflect the diversity of endophytic fungi in Ginkgo tissue specificity. Above 178 fungal fermentation broth of esters TLC test found 36 ginkgolide C spots color R_f the value approaching. These 36 fermentation broth inhibits platelet aggregation experiments, and found that 14 can effectively inhibit ADP-induced platelet aggregation, one of the seven is considered to be able to produce ginkgolide C, or structural analogues thereof. The first time the 48 endophytic fungi isolated from two different sources Magnolia stems identified by morphological observation and 1 class, 3 orders, 6 families, 16 genera. Purpose classification level the Cong Terrier spore head (Moniliales) was the dominant population; the dominant species of the genus Aspergillus (Aspergillus), the genus Penicillium (Penicillium) and Fusarium (Fusarium) in the case of the classification level. Different sources Magnolia endogenous fungal population and species distribution. 27 in respect of the endophytic fungi magnolol and honokiol HPLC detection, and found 17 fungal hyphae or fermentation broth containing honokiol and magnolol or its structural analogues. Another 27 fungal fermentation broth of Staphylococcus aureus (Staphylococcus aureus), E. coli (Escherichia coli), Pseudomonas aeruginosa (Pseudomonas aeruginosa) human pathogenic bacteria antibacterial experiments, the results show that the 7 fungal fermentation solution of Staphylococcus aureus (Staphylococcus aureus), Escherichia coli (Escherichia coli) have different degrees of inhibition, and rescreening using different media, select a strong inhibitory effect of FM044 antibacterial substances from basic research. Isolated from a white crystal, via IR, MS, ~ 1H-NMR spectral analysis of D-mannitol (with mannitol). Antibacterial substances from basic research is still in progress.