Effect of Human Recombinant PLD2 in Vivo on Serumal GPI-PLD as Well as the Expression of Nuclear Transcription Factor P65 in Chronic Asthma of Guinea Pigs
|Keywords||Human recombinant phospholipase D2 GPI-PLD Nuclear transcription factor P65 Chronic asthmatic guinea pigs|
Objective: through the establishment of a chronic asthma model in guinea pigs , the study of human recombinant phospholipase the D2 (rhPLD2) after the intervention of chronic asthma in guinea pigs , its serum glycosylated phosphatidylinositol - specific phospholipase D (GPI-PLD) activity, lung tissue changes in the content of the nuclear transcription factor P65 , and to explore the possible mechanism . Method : to is rhPLD2 that intervention in chronic asthmatic guinea pigs , guinea pig serum GPI-PLD activity of TX-114 phase separation method detects ; TransAM Transcription Factor Assay Kits kit detection of lung tissue nuclear transcription factor P65 . Results: ① normal state of guinea pig compared to chronic asthma status guinea pig serum GPI-PLD activity of lung tissue nuclear transcription factor P65 in have significantly increased in . Dexamethasone chronic asthmatic guinea pigs ( 2 ) to rhPLD2 and ground , the two indexes have different degrees of decline. The Conclusion : rhPLD2 may suppress inflammatory response in asthma by inhibiting the secretion of GPI-PLD expression and organization of the nuclear transcription factor P65 .