Dissertation > Biological Sciences > Zoology > Animal evolution and development > Evolution of animals and animal phylogeny

Characterization, Expression and Evolution of AmphiANT and AmphiTRAPβ Genes from Amphioxus

Author XiaJianJun
Tutor ZhangShiZuo
School Ocean University of China
Course Genetics
Keywords Amphioxus Adenine nucleotide transporter AmphiANT Easy seat related protein AmphiTRAP β Phylogenetic analysis Expression pattern
CLC Q951.3
Type Master's thesis
Year 2005
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Amphioxus, between transition type animals between vertebrates and invertebrates. Using molecular biology methods to study the structure of amphioxus gene evolution and expression, can reveal the mechanism of amphioxus development of molecular biology to help us understand the origin and evolution of vertebrates from the molecular level up. Currently, the the amphioxus gene sequences and expression patterns have been widely used in the comparison between the different species of genomics research and developmental homology analysis. This paper first reported the amphioxus (Branchiostoma belcheri tsingtauense) intestinal cDNA library in AmphiANT, and AmphiTRAPβ gene cloning and identification, and their structure, system evolution and expression patterns. AmphiANT full-length 1289 bps, containing a 897bps open reading frame encoding a 298 amino acid protein consisting of. Protein encoded by this gene by NCBI retrieval a Blastp tools on the similarity of the protein with ANT protein, zebrafish and Drosophila ANT corresponding protein was 74%, 76% and 74%, respectively. The sequence comparison analysis revealed that it has for ANT the recognition sequence RRRMMM, while having three sections each include about 100 amino acid sequence homology, and each has a mitochondrial carrier protein has a PX-[D / E]-XX-[ R / K] yl sequence. Therefore, the gene is considered encoding amphioxus ANT protein, named AmphiANT. Phylogenetic analysis through gene AmphiANT system found The amphioxus AmphiANT in the middle of the two branches of vertebrate and invertebrate alone into a. Northern blot analysis showed that in the the adult amphioxus organization, AmphiANI. Were expressed, but ovarian AmphiANT the expression is especially rich; great changes expression of AmphiANT amphioxus embryonic development, hatched larvae and larvae a day rich in eggs, neurula, the zygote, capsule weak expression in the embryo and gastrulation. ANT abundantly expressed in the amphioxus development process should be associated with the process of the development of a large number of energy demand. The AmphiTRAPβ full length 687 bps contains an open reading frame of a 561bps, encoding a 187 amino acid residues consisting of a molecular weight of 20.4 kDa protein. This gene encodes a protein, Retrieving the Blastp tool on NCBI, found of this protein TRAPβ protein have high similarity with people, the dogs and zebrafish corresponding TRAPβ protein similarity were 56%, 59% and 60%. The sequence comparison analysis revealed that it has the characteristics of the TRAPβ, there are two conserved can be glycosylated Asn site and a transmembrane segment; amphioxus transmembrane region with human, dog, and zebrafish TRAPβ protein transmembrane region identical . The two evolutionary analysis tree constructed using maximum parsimony and temporary knot that amphioxus AmphiTRAPβ between vertebrates and invertebrates

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