Dissertation
Dissertation > Biological Sciences > Microbiology > Microbial Genetics

Studies on NSP Enzymes Produced by Trichoderma Reesei Rut C-30 and Improving Xylanase Activity by UV Mutation

Author LeYiLin
Tutor ZengZheLing;LiSiGuang;XiongTao;ChengChi
School Nanchang University
Course Microorganism
Keywords Trichoderma reesei Rut C-30 Non- starch polysaccharide enzyme UV Mutation Breeding Xylanase
CLC Q933
Type Master's thesis
Year 2005
Downloads 157
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Trichoderma reesei Rut C-30 as the starting strain , its non- starch polysaccharides enzymes were analyzed by UV Mutation breeding superior strains produced xylanase . Trichoderma reesei Rut C-30 to the straw and wheat bran as a substrate for solid state fermentation by different carbon sources and add different inducer their cellulase , xylanase , beta - glucanase of β- mannanase , pectinase five different non- starch polysaccharide enzyme analysis, the results showed that the Trichoderma reesei Rut C - 30 produced by non- starch polysaccharide enzymes than the whole , different carbon sources and add different inducer solid fermentation, can change the proportion of the fermentation process to produce the non-starch polysaccharide enzymes . Trichoderma reesei Rut C-30 the fresh spores by ultraviolet radiation , mutation breeding, breeding superior strains produced xylanase . Selection of fast-growing colony screening medium containing xylan as rescreening strain , and then inoculated into 250mL flask solid fermentation medium for rescreening . Screened strains , its enzymatic activity than the starting strain increased 41.9% . The proportion of carbon, nitrogen, dry material with water , incubation time , incubation temperature and inorganic salts factors bred strains fermentation conditions . The results show that the best straw and wheat bran ratio of 6:4 ; optimum nitrogen source was ammonium sulfate , 2.0% of the amount added the highest activity ; optimum ratio of 1:3.0 dry material with water ; orthogonal experiments show incubation time the greatest impact on the production of enzymes , followed by the the KH 2 PO 4 , and MgSO 4 · 7H 2 O incubation temperature on the enzyme activity affecting most times .

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