Dissertation
Dissertation > Medicine, health > Chinese Medicine > Of Pharmacy > Pharmacology

Growth Inhibition and Demethylation by a Component of Natural Drug, CDP, in Cancer Cell Lines

Author ZuoJu
Tutor ZuoYang
School Sichuan University
Course Biochemistry and Molecular Biology
Keywords Chinese medicines CDP 5-aza-cytidine Demethylation Human hepatoma cell line Human breast cancer cell lines p16 gene
CLC R285
Type Master's thesis
Year 2005
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Background and Purpose Epigenetics refers to changes in gene expression of the gene sequence does not change the case , including DNA methylation , RNA silencing and histone modifications . It is generally agreed that the promoter CpG island hypermethylation leading to gene mutations, deletions , and other genetic mechanisms , tumor suppressor genes and tumor-associated gene inactivation another mechanism , such as p16 , GSTPl and E -cadherin . After DNA replication , cytosine methylation occurs faster : DNA methyltransferase enzyme ( DNA methyltransferases , DNMTs ) under the action of the transfer of the methyl group from S-adenosyl homocysteine ??(S-adenosylmethionine, SAM) to the cytosine in the 5-position carbon atom , form a 5 - methylcytosine ( 5mC ) . Go methyltransferase inhibitor 5 -aza -cytidine ( 5 -aza - C ) and of decitabine by and DNMTs forming covalent binding , cytosine can not occur on the sub- strand DNA methylation , the reversal of a tumor suppressor gene and tumor-associated high methylation status of the gene , the silence gene expression . But its strong mutagenicity , cytotoxicity , and side effects such as bone marrow suppression limited clinical application . Our previous study , Chinese medicines CDP as analogues of SAM , demethylation , we will further study the inhibition of the growth of a variety of tumor cell lines and reversal of the p16 gene promoter region CpG island methylation the role of the state . Cultured human hepatoma cell line in Huh - 7 and breast cancer cell lines T47D, MCF-7 and

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