Basic and Clinical Studies on the Effects of Peroxisome Proliferator-activated Receptor γ on Biological Behaviors of Human Colorectal Cancer
|Course||Digestion within the science|
|Keywords||Apoptosis Cell cycle PPARγ PTEN Tissue microarray Immunohistochemical method Colorectal cancer|
Objective To study the first part of the peroxisome proliferator-activated receptor γ expression and its clinical significance in colorectal carcinoma tissue peroxisome proliferator- activated receptor γ (peroxisome proliferator-activated reccptorγ, PPARγ) and PTEN (phosphatase and relationship between tensin homologue deleted on chromosome ten) expression in colorectal cancer and their clinical and pathological data , explore its possible biological significance . Methods 139 cases of colorectal cancer , 18 cases of colorectal adenomas , 50 cases of cancer and adjacent normal mucosa paraffin-embedded tissue using tissue microarray instrument made ??with 104 points and 72 points two colon tissue chip and 130 and 54 points two The rectal tissues chip . Using immunohistochemistry Law PPARγ and PTEN expression was detected in the tissue microarray . Results of the structural integrity of the tissue microarray , each lattice are representative morphology observed after staining . The immunohistochemistry result of PPARγ and PTEN expression in colorectal adenomas and normal colorectal tissue no difference (P = 0.207 , P = 0.250 ) . PTEN expression and colorectal cancer histological degree (P = 0.006) and distant metastasis (P = 0.015) . Conclusion tissue microarray as a new molecular biology techniques in the the tumor pathological study will play an important role . PPARγ and PTEN expression may not change in the incidence of colorectal cancer process , may not be able to detect the protein expression of PPARγ and PTEN as one of the early diagnosis of colorectal cancer . But the low expression of PTEN or missing predictors may be malignant differentiation and metastasis of colorectal cancer , and may contribute to the prognosis .