Dissertation > Medicine, health > Oncology > Gastrointestinal Cancer > Intestinal neoplasms > Colorectal tumors

Basic and Clinical Studies on the Effects of Peroxisome Proliferator-activated Receptor γ on Biological Behaviors of Human Colorectal Cancer

Author LinMaoSong
Tutor ChenWeiChang
School Suzhou University
Course Digestion within the science
Keywords Apoptosis Cell cycle PPARγ PTEN Tissue microarray Immunohistochemical method Colorectal cancer
CLC R735.34
Type Master's thesis
Year 2005
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Objective To study the first part of the peroxisome proliferator-activated receptor γ expression and its clinical significance in colorectal carcinoma tissue peroxisome proliferator- activated receptor γ (peroxisome proliferator-activated reccptorγ, PPARγ) and PTEN (phosphatase and relationship between tensin homologue deleted on chromosome ten) expression in colorectal cancer and their clinical and pathological data , explore its possible biological significance . Methods 139 cases of colorectal cancer , 18 cases of colorectal adenomas , 50 cases of cancer and adjacent normal mucosa paraffin-embedded tissue using tissue microarray instrument made ??with 104 points and 72 points two colon tissue chip and 130 and 54 points two The rectal tissues chip . Using immunohistochemistry Law PPARγ and PTEN expression was detected in the tissue microarray . Results of the structural integrity of the tissue microarray , each lattice are representative morphology observed after staining . The immunohistochemistry result of PPARγ and PTEN expression in colorectal adenomas and normal colorectal tissue no difference (P = 0.207 , P = 0.250 ) . PTEN expression and colorectal cancer histological degree (P = 0.006) and distant metastasis (P = 0.015) . Conclusion tissue microarray as a new molecular biology techniques in the the tumor pathological study will play an important role . PPARγ and PTEN expression may not change in the incidence of colorectal cancer process , may not be able to detect the protein expression of PPARγ and PTEN as one of the early diagnosis of colorectal cancer . But the low expression of PTEN or missing predictors may be malignant differentiation and metastasis of colorectal cancer , and may contribute to the prognosis .

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